UV-1100 spectrophotometric sodium benzoate detection - Database & Sql Blog Articles

Determination of Sodium Benzoate by Spectrophotometry

This experiment focuses on the determination of sodium benzoate using spectrophotometric methods. The key objective is to analyze the concentration of sodium benzoate in carbonated beverages accurately. Sodium benzoate (C₆H₅COONa) is a widely used food preservative known for its ability to prevent microbial growth and extend shelf life. However, concerns about its potential toxicity have led to restrictions in some countries. Sodium benzoate is a white crystalline powder, slightly soluble in water, and becomes more effective when converted into benzoic acid under acidic conditions. In this study, we compare two methods: biphasic titration and UV-Vis spectrophotometry. The spectrophotometric method involves preparing a standard curve by measuring absorbance at 223 nm, which allows for accurate quantification of sodium benzoate in samples. The experimental setup includes an UV-1300 spectrophotometer, glassware such as beakers, flasks, and pipettes, as well as reagents like hydrochloric acid, sodium hydroxide, and potassium hydrogen phthalate. The sample preparation process involves removing CO₂ from the beverage, extracting the compound, and then analyzing it using the spectrophotometric technique. The results are compared with those obtained through titration to ensure accuracy. This method is not only efficient but also suitable for routine analysis in food laboratories. It provides reliable data that can help in quality control and regulatory compliance. By understanding the properties and behavior of sodium benzoate, researchers and food scientists can better assess its safety and effectiveness in various applications. Keywords: spectrophotometry; food additive; analytical instrument; UV-1300.