Alkaline phosphatase (ALP) polyclonal antibody detection instructions - Database & Sql Blog Articles

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Alkaline Phosphatase (ALP) Polyclonal Antibody

Polyclonal Antibody to Alkaline Phosphatase (ALP) - AKP; ALKP; Basic Phosphatase For in vitro studies only, not for clinical diagnosis! Please request an import duty tax return and customs declaration form! Bos taurus; Bovine (Cattle, cattle) brand RD BIM ABCAM. (USA) Source: polyclonal antibody preparation, host: rabbit, titer: - Ig type: IgG, Purification method: antigen-specific affinity purification, label: label-free, immunogen: - buffer component: Phosphate buffer (pH 7.4, containing 0.02% NaN3 and 50% glycerol), trait: liquid, concentration: 200ug/ml, suitable species: - application: WB, ICC, IHC-P, IHC-F, ELISA. Download English manual, Chinese manual. Specification: 10ug, 50ug, 100ug, 1mg, 1g.

Organism speciesHomo sapiens (Human)
Product No.yb472Hu01
ClonalityPolyclonal
HostRabbit
Immunoglobulin TypeIgG
PurificationAffinity Chromatography
ApplicationsWB, ICC, IHC-P, IHC-F, ELISA
Concentration200ug/ml
UOM100ug
ConjugateNo Conjugate

Certificate
  1. 1

Passed ISO 9001, ISO 13485 quality system certification

Specificity

This antibody is a rabbit polyclonal antibody against ALP. It has been validated to detect ALP in immunohistochemical staining and western blotting experiments. The antibody shows high specificity and minimal cross-reactivity with other proteins.

Usage

Western blot: 1:50–400 Immunocytochemistry: 1:50–500 Immunohistochemistry (frozen section): 1:50–500 Immunohistochemistry (paraffin section): 1:10–100 ELISA: 1:100–1:5000 The optimal dilution factor should be determined by the user based on their experimental conditions.

Storage

Store at 4°C for short-term use. For long-term storage, keep at -20°C. Avoid repeated freeze-thaw cycles to maintain stability and activity of the antibody.

Stability

The antibody demonstrates good thermal stability. It can withstand incubation at 37°C for 48 hours without significant degradation or precipitation. Under proper storage conditions, the loss rate during the warranty period is less than 5%.

Shanghai Jinma has been providing ELISA kits and related reagents for many years. Our team offers comprehensive pre-sale, sales, and after-sales support to ensure your experiments are successful. We appreciate your trust and support. Wishing you great success in your research!

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