How to determine the results of ELISA kit experiments - Database & Sql Blog Articles

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When you're ready to finish your ELISA experiment, it's important to know how to interpret the results. ELISA tests can provide quantitative, semi-quantitative, or qualitative data. Let’s go through each one in detail.

1. Quantitative Determination: This method involves using a known standard and performing serial dilutions. The sample is tested alongside the standard, and a standard curve is plotted. The result is then expressed in absolute values or active units, making it highly precise for measuring concentrations.

2. Semi-Quantitative Determination: In this approach, results are usually expressed as titers. After serial dilution of the sample, an ELISA is performed. The highest dilution that still shows a positive reaction above the cutoff value is considered the titer, which gives an approximate measure of the concentration.

3. Qualitative Determination: This method helps determine whether a sample is positive or negative. There are two common approaches:

(a) Cut-off Value Method: A cut-off (CO) value is calculated, typically by adding a constant to the average absorbance of the negative control. If the sample's absorbance is equal to or higher than the CO value, it's considered positive. This method requires strict reagent preparation and accurate calibration.

(b) Inhibition Rate Method: Used in competitive ELISA, this method calculates the inhibition rate. The formula is: Inhibition Rate (%) = [(A of Negative Control - A of Sample) / A of Negative Control] × 100. Generally, a result with an inhibition rate of 50% or higher is considered positive.

Understanding these methods will help you accurately interpret your ELISA results. Whether you're conducting research, diagnostics, or quality control, choosing the right ELISA kit is essential. If you're looking for reliable and cost-effective solutions, consider purchasing from Shanghai Hengyuan. We offer high-quality kits at competitive prices. Feel free to reach out for more information and support.